Reporter

Part:BBa_K3020004:Design

Designed by: BIT   Group: iGEM19_BIT   (2019-10-04)


umuDC+gfp


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1091


Design Notes

You need to use the proper annealing temperature in the PCR.


Source

The promoter umuDC is from Escherichia coli str. K-12 substr. MG1655.

References